EVIDENCE FOR THE PRESENCE OF A LOW MR GTP-BINDING PROTEIN, RAM P25, IN HUMAN PLATELET MEMBRANES

被引：6
作者：
NAGATA, K [1 ]
OKANO, Y [1 ]
SUZUKI, T [1 ]
NOZAWA, Y [1 ]
机构：
[1] GIFU UNIV,SCH MED,DEPT BIOCHEM,TSUKASAMACHI 40,GIFU 500,JAPAN
来源：
PLATELETS | 1993年 / 4卷 / 05期
关键词：
D O I：
10.3109/09537109309013227
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The ram gene was isolated from a rat megakaryocyte cDNA library and encodes a GTP-binding protein with a Mr of 25 000. In order to clarify the presence of the ram protein (ram p25) in human platelets, we tried to purify ram p25 from the sodium cholate extract of human platelet membranes by a combination of DEAE-Sephacel, Sephacryl S300HR, hydroxyapatite HCA-100S and DEAE-Toyopearl 650(S) column chromatographies. In the course of the purification, a specific antibody raised against a synthetic COOH-terminal peptide of ram p25 was used. In conclusion, ram p25 was partially purified and was observed to be present in membrane fractions of human platelets.
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页码：268 / 274
页数：7
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