A one-factor field experiment established by means of random block sampling, in four replicants, was located on the degraded chernozem with loess subsoil. The kind of fertilization with microelements was an assessed factor. Foliar fertilizers were applied in the form of single microelements (copper, zinc, manganese) and the multicomponent formulation Plonvit P containing elements in the form of chelates. It was found that applied fertilization had the most spectacular effect on the magnesium content in timothy grass. As a result of foliar fertilization with the multicomponent formulation, copper and manganese, significant increase of the average content of this element was observed (by 52 %, 79 %, 85 %, respectively). Moreover, it was determined that foliar fertilization with the examined microelements caused elevation of the calcium and potassium level by 31 % and 22 % (respectively) when compared with the control object. Fertilization with copper had a negative effect on the phosphorus content, whereas application of manganese negatively influenced the level of sodium. Fertilization with the multicomponent fertilizer, copper and manganese narrowed proportions between the sum of univalent and divalent cations in timothy grass.