USE OF A SILVER-ENHANCED GOLD-LABELED IMMUNOASSAY FOR DETECTION OF ANTIBODIES TO THE HUMAN-IMMUNODEFICIENCY-VIRUS IN WHOLE-BLOOD SAMPLES

被引：8
|
作者：
ROCKS, BF
PATEL, N
BAILEY, MP
机构：
[1] Biochemstry Deparment, Royal Sussex County Hospital, Brighton BN2 5BE, Eastern Road
来源：
ANNALS OF CLINICAL BIOCHEMISTRY | 1991年 / 28卷
关键词：
AIDS SERODIAGNOSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY;
D O I：
10.1177/000456329102800206
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
A silver-enhanced gold-labelled immunosorbent assay (SEGLISA) for the detection of antibodies to the immunodeficiency virus (HIV) in whole-blood samples is described. This new non-isotopic, non-enzymic immunoassay incorporates use of solid phase viral antigens which bind any HIV antibodies present in the test sample. The antigen/antibody complex is then detected by gold-labelled anti human immunoglobulin G (IgG) followed by silver amplification. We found that whole blood samples give false positives when using a horseradish peroxidase label, whereas the SEGLISA correctly identified 50 HIV antibody positive samples and 50 HIV antibody negative samples when using whole blood. The use of whole blood collected on filter paper is also described. The SEGLISA has good precision (CV = 7.5%) and sensitivity.
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页码：155 / 159
页数：5
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