L-ASPARAGINASE-II OF ESCHERICHIA-COLI K-12 - CLONING, MAPPING AND SEQUENCING OF THE ANSB GENE

被引：25

作者：

BONTHRON, DT

机构：

[1] Human Genetics Unit, Department of Medicine, Western General Hospital, Edinburgh

来源：

GENE | 1990年 / 91卷 / 01期

关键词：

Bacterial genetics; leukaemia; periplasmic; physical map; recombinant DNA;

D O I：

10.1016/0378-1119(90)90168-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Escherichia coli gene ansB, encoding the chemotherapeutic enzyme l-asparaginase II, has been cloned, using a strategy based on the polymerase chain reaction, and sequenced. The amino acid (aa) sequence differs in eleven positions from the data previously derived by direct aa sequencing. A cleavable secretory signal peptide precedes the N terminus of the mature protein. The ansB gene maps to position 3114 kb on the physical map of E. coli [Kohara et al., Cell 50 (1987) 495-508], corresponding to approx. 63.8 min on the genetic map. © 1990.

引用

页码：101 / 105

页数：5

共 34 条

[1]

ALLEN G, 1981, SEQUENCING PROTEINS, P171

[2] LINKAGE MAP OF ESCHERICHIA-COLI-K-12, EDITION-7 [J].