LIPOPROTEINS CONTAINING APOLIPOPROTEIN A-IV - COMPOSITION AND RELATION TO CHOLESTEROL ESTERIFICATION

被引：15
作者：
DUVERGER, N [1 ]
GHALIM, N [1 ]
THERET, N [1 ]
FRUCHART, JC [1 ]
CASTRO, G [1 ]
机构：
[1] INST PASTEUR, INSERM, U325, F-59019 LILLE, FRANCE
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1211卷 / 01期
关键词：
LIPOPROTEIN; APO A-I; APO A-II; APO A-IV; LCAT; CHOLESTEROL ESTERIFICATION; SPHINGOMYELIN; PHOSPHATIDYLCHOLINE; FATTY ACID COMPOSITION;
D O I：
10.1016/0005-2760(94)90134-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to investigate the relationship of lipid and apolipoprotein composition to cholesterol esterification in lipoproteins containing apolipoprotein (ape) A-IV, apo A-containing lipoprotein particles were isolated from fresh human plasma using a system of sequential immunoaffinity chromatography. Plasma was first depleted of apo B- and apo E-containing lipoproteins. Four major subpopulations of ape A-containing lipoprotein particles were separated: Lp A-I, Lp A-I:A-II, Lp A-IV and Lp A-I:A-IV:A-II. Lp A-IV and Lp A-I:A-IV:A-II contained less total lipid, less cholesterol and more triacylglycerol than Lp A-I and Lp A-I:A-II. Lp A-IV and Lp A-I:A-IV:A-II contained more sphingomyelin and less phosphatidylcholine than Lp A-I and Lp A-I:A-II and were richer in (16:0 +/- 18:0) saturated fatty acids. Among these isolated lipoprotein particles, Lp A-IV contained the highest lecithin: cholesterol acyltransferase (LCAT) activity per mu g of protein. Cholesterol esterification rates were 2.6 +/- 0.5, 5.3 +/- 0.4 and 0.8 +/- 0.2 mu mol of cholesterol per hour per m,a of lipoproteins for Lp A-IV, Lp A-I and Lp A-I:A-II, respectively. The apolipoprotein and lipid composition and LCAT activity of Lp A-IV suggest that this lipoprotein may be a source of cholesterol esterification in plasma.
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页码：23 / 28
页数：6
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