IDENTIFICATION OF ORAL YEASTS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：8
|
作者：
MAIDEN, MFJ
TANNER, A
机构：
[1] Forsyth Dental Center, Boston, Massachusetts
来源：
ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 03期
关键词：
ORAL YEAST; CANDIDA-ALBICANS; SDS-PAGE; IDENTIFICATION;
D O I：
10.1111/j.1399-302X.1991.tb00475.x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Protein profiles of sonicated cells for 9 species of yeasts isolated from oral samples were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels were distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS-PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests.
引用
收藏
页码：187 / 190
页数：4
相关论文
共 50 条
12345