THE USE OF FLUORESCENT-PROBES IN IMMUNOCHEMISTRY

被引：48
作者：
DAVIDSON, RS
HILCHENBACH, MM
机构：
[1] Department of Chemistry, City University, London, EC1V OHB, Northampton Square
来源：
PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1990年 / 52卷 / 02期
关键词：
D O I：
10.1111/j.1751-1097.1990.tb04200.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：431 / 438
页数：8
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