CRYSTALLIZATION OF A HUMICOLA-LANUGINOSA LIPASE-INHIBITOR COMPLEX WITH THE USE OF POLYETHYLENE-GLYCOL MONOMETHYL ETHER

被引：28

作者：

BRZOZOWSKI, AM ^{[1
]}

机构：

[1] UNIV LODZ,DEPT CRYSTALLOG,PL-90236 LODZ,POLAND

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1993年 / 49卷

关键词：

D O I：

10.1107/S0907444993000952

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The fungal Humicola lanuginosa lipase complexed with the inhibitor n-dodecylphosphonate ethyl ester was crystallized in space group P2(1)2(1)2(1) with pseudotetragonal unit-cell parameters of a = 131.7 (2), b = 131.3 (1), c = 75.4 (1) angstrom. 92% of X-ray diffraction data to 2.8 angstrom resolution were collected with a final R(merge) = 8.5%. The crystals were grown using a new kind of precipitant - polyethylene glycol monomethyl ether (Peg-mme) of molecular weight 5000.

引用

页码：352 / 354

页数：3

共 8 条

[1] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].