ELECTRO-PHYSIOLOGICAL RECORDINGS FROM SPONTANEOUSLY CONTRACTING REAGGREGATES OF CULTURED SMOOTH-MUSCLE CELLS FROM GUINEA-PIG VAS-DEFERENS

被引:11
作者
MCLEAN, MJ
PELLEG, A
SPERELAKIS, N
机构
关键词
D O I
10.1083/jcb.80.3.539
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Smooth muscle cells were enzymatically dispersed from vasa deferentia of adult male guinea pigs (250-400g). These cells reassociated in vitro to form monolayers and small spherical reaggregates (0.05-0.3 mm in Diam). Within 48 h of being placed in culture, cells in both types of preparation began to contract spontaneously. The contractions were rhythmic and slow. Cells in the monolayers stopped contracting after - 1 wk in vitro, but the reaggregates continued to contract spontaneously for at least 3 wk. Electron microscopy of the reaggregates revealed the presence of thick and thin myofilaments. Overshooting action potentials were recorded in many of the cells penetrated (primarily in reaggregates), and were accompanied by visible contractions of the aggregate or monolayer. Quiescent cells could often be excited by intracellularly applied depolarizing and hyperpolarizing (anodal-break) current pulses. The resting potentials had a mean value of -58 ± 2mV. The action potentials were usually preceded by a spontaneous depolarization. The action potentials had slow rates of rise (1-4 V/s) which were unaffected by tetrodotoxin (TTX, 1 μg/ml), a known blocker of fast NA+-channels. Verapamil (1μg/ml) blocked the action potentials. The mean value of input resistance was 6.9 ± 0.5 MΩ (n= 12). These electrophysiological properties are similar to those of intact adult vas deferens smooth muscle cells. Thus, the cultured adults vas deferens smooth muscle cells retrain their functional properties in vitro even after long periods.
引用
收藏
页码:539 / 552
页数:14
相关论文
共 50 条
[41]   MECHANISM OF ENHANCEMENT BY SMOOTH-MUSCLE STIMULANTS OF MOTOR RESPONSES OF GUINEA-PIG VAS-DEFERENS TO NERVE-STIMULATION [J].
SJOSTRAND, NO ;
SWEDIN, G .
ACTA PHYSIOLOGICA SCANDINAVICA, 1974, 90 (03) :513-521
[42]   REQUIREMENTS OF EXTERNAL CA AND NA FOR THE ELECTRICAL AND MECHANICAL RESPONSES TO NORADRENALINE IN THE SMOOTH-MUSCLE OF GUINEA-PIG VAS-DEFERENS [J].
WAKUI, M ;
INOMATA, H .
JAPANESE JOURNAL OF PHYSIOLOGY, 1984, 34 (01) :199-203
[43]   EFFECTS OF PHTHALAZINOL ON SMOOTH-MUSCLE CELLS OF THE PORCINE CORONARY-ARTERY AND ON NEUROMUSCULAR-JUNCTION ON THE GUINEA-PIG VAS-DEFERENS [J].
KITAMURA, K ;
TAJIMA, K ;
KAMATA, M ;
ITO, Y ;
KURIYAMA, H .
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1980, 311 (02) :159-167
[44]   COMPARISON OF ELECTRICAL-PROPERTIES AND MORPHOLOGICAL-CHARACTERISTICS OF SMOOTH-MUSCLE OF RAT AND GUINEA-PIG VAS-DEFERENS [J].
GOTO, K ;
MILLECCHIA, LL ;
WESTFALL, DP ;
FLEMING, WW .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1977, 368 (03) :253-261
[45]   COMPARISON OF MECHANICAL-PROPERTIES OF SMOOTH-MUSCLE IN A STRIP PREPARATION AND A WHOLE TISSUE OF GUINEA-PIG VAS-DEFERENS [J].
MIZOBE, H ;
TSUCHIYA, T ;
SUGI, H .
ZOOLOGICAL SCIENCE, 1985, 2 (06) :886-886
[46]   EFFECT OF A SMOOTH-MUSCLE RELAXANT FROM THE STONEFISH, SYNANCEIA-TRACHYNIS, ON KCL-INDUCED RESPONSES IN THE GUINEA-PIG VAS-DEFERENS [J].
HOY, AWW ;
CAMERON, AM .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 129 (1-2) :105-112
[47]   DILTIAZEM ACTIONS ON SMOOTH-MUSCLE CELLS OF THE PORCINE CORONARY-ARTERY AND ON NEUROMUSCULAR-JUNCTIONS OF THE GUINEA-PIG VAS-DEFERENS [J].
TAJIMA, K ;
KANDA, S ;
KITAMURA, K ;
ITO, Y ;
KURIYAMA, H .
GENERAL PHARMACOLOGY, 1980, 11 (06) :561-568
[48]   ELECTRO-PHYSIOLOGICAL STUDY OF THE ACTION OF NEUROTENSIN ON THE SMOOTH-MUSCLE OF THE GUINEA-PIG TAENIA-COLI [J].
KITABGI, P ;
HAMON, G ;
WORCEL, M .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1979, 56 (1-2) :87-93
[49]   FACILITATION OF TRANSMISSION FROM AUTONOMIC NERVE TO SMOOTH MUSCLE OF GUINEA-PIG VAS DEFERENS [J].
BURNSTOCK, G ;
KURIYAMA, H ;
HOLMAN, ME .
JOURNAL OF PHYSIOLOGY-LONDON, 1964, 172 (01) :31-&
[50]   INWARD CURRENTS RECORDED ON P-2X-PURINOCEPTOR ACTIVATION IN GUINEA-PIG VAS-DEFERENS ISOLATED SMOOTH-MUSCLE CELLS [J].
PIPER, AS ;
HOLLINGSWORTH, M .
BRITISH JOURNAL OF PHARMACOLOGY, 1995, 116 :P268-P268