A PRELIMINARY REPORT ON INTERMEDIATES IN CONVERSION IN VITRO OF CHOLESTEROL TO PREGNENOLONE IN ADRENAL PREPARATIONS

被引：30

作者：

BURSTEIN, S

GUT, M

机构：

[1] Division of Steroid Chemistry, Institute for Muscle Disease, Inc., New York

[2] The Worcester Foundation for Experimental Biology Shrewsbury

来源：

STEROIDS | 1969年 / 14卷 / 02期

关键词：

D O I：

10.1016/0039-128X(69)90034-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reaction sequences cholesterol → 20α-hydroxycholesterol → 20α, 22R-di-hydroxycholesterol → pregnenolone, and cholesterol → 22R-hydroxycholesterol → 20α, 22Rrdihydroxycholesterol → pregnenolone have been studied quantitatively under conditions of first-order kinetics with adrenocortical preparations. The sequence of 22R-hydroxycholesterol was much more important than that of 20α-hydroxycholesterol, but only a small fraction of the pregnenolone formation from cholesterol via the monohydroxy derivatives could be accounted for. A much more substantial fraction could be accounted for by assuming an enzymatic, concerted attack of oxygen on cholesterol to form 20α, 22R-dihydroxycholesterol which then is oxidatively cleaved to pregnenolone. This study establishes several pathways leading from cholesterol to pregnenolone. © 1968.

引用

页码：207 / &

共 18 条

[1]

BURSTEIN S, 1968, J BIOL CHEM, V243, P4417

[2]

BURSTEIN S, 1969, FED PROC, V28, P666

[3]

BURSTEIN S, 1967, EXCERPTA MED INT 132, P437

[4]

CHAUDHURI AC, 1962, J BIOL CHEM, V237, P703

[5] CLEAVAGE OF CHOLESTEROL SIDE CHAIN BY ADRENAL CORTEX .3. IDENTIFICATION OF 20 ALPHA-22 XI-DIHYDROXYCHOLESTEROL AS AN INTERMEDIATE [J].