STUDIES WITH ALPHA-KETOGLUTARATE DEHYDROGENASE MUTANTS OF ESCHERICHIA COLI

被引：38
作者：
HERBERT, AA
GUEST, JR
机构：
[1] Department of Microbiology, University of Sheffield
来源：
MOLECULAR AND GENERAL GENETICS | 1969年 / 105卷 / 02期
关键词：
D O I：
10.1007/BF00445687
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two classes of mutant lacking α-ketoglutarate dehydrogenase complex activity were detected by biochemical analysis of strains of Escherichia coli requiring succinate for aerobic growth on glucose minimal medium. One class, designated sucA, lacked the α-ketoglutarate decarboxylase component (E1) whereas the other class, sucB, lacked the dihydrolipoyl transsuccinylase component (E2). Studies with mixed cell-free extracts showed that the overall dehydrogenase activity could be reconstituted from several pairs of sucA plus sucB mutants but not from mixtures of mutants of the same class. Transduction analysis with phage P1 indicated close linkage between the two genes and their frequencies of cotransduction with gal were similar. The order of the two genes was also established as sucA (E1)-sucB(E2)... gal by reciprocal three-point crosses with several pairs of mutants. © 1969 Springer-Verlag.
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页码：182 / &
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