L-MONOCYTOGENES-INDUCED ACTIN ASSEMBLY REQUIRES THE ACTA GENE-PRODUCT, A SURFACE PROTEIN

被引：661
作者：
KOCKS, C
GOUIN, E
TABOURET, M
BERCHE, P
OHAYON, H
COSSART, P
机构：
[1] INST PASTEUR,CENT MICROSCOPIE ELECTR STN,F-75724 PARIS 15,FRANCE
[2] UNIV PARIS 05,MICROBIOL LAB,F-75730 PARIS 15,FRANCE
来源：
CELL | 1992年 / 68卷 / 03期
关键词：
D O I：
10.1016/0092-8674(92)90188-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to cell without leaving the cytoplasm. The mechanism of this movement, generated through bacterially induced actin polymerization, is not understood. By analyzing an avirulent Tn917-lac mutant defective for actin polymerization, we have identified a bacterial component involved in this process. The transposon had inserted in actA, the second gene of an operon. Gene disruption of downstream genes and transformation of the mutant strain with actA showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly. Our results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd.
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页码：521 / 531
页数：11
相关论文
共 48 条
[1]   IDENTIFICATION OF ICSA, A PLASMID LOCUS OF SHIGELLA-FLEXNERI THAT GOVERNS BACTERIAL INTRA-CELLULAR AND INTERCELLULAR SPREAD THROUGH INTERACTION WITH F-ACTIN [J].
BERNARDINI, ML ;
MOUNIER, J ;
DHAUTEVILLE, H ;
COQUISRONDON, M ;
SANSONETTI, PJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3867-3871
[2]   INTRACELLULAR PROTEIN TOPOGENESIS [J].
BLOBEL, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1496-1500
[3]  
BRYAN J, 1989, J BIOL CHEM, V264, P13873
[4]  
COSSART P, 1989, MOL BIOL MED, V6, P463
[5]   CDNA-DERIVED SEQUENCE OF CHICKEN-EMBRYO VINCULIN [J].
COUTU, MD ;
CRAIG, SW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8535-8539
[6]   LISTERIA-MONOCYTOGENES MOVES RAPIDLY THROUGH THE HOST-CELL CYTOPLASM BY INDUCING DIRECTIONAL ACTIN ASSEMBLY [J].
DABIRI, GA ;
SANGER, JM ;
PORTNOY, DA ;
SOUTHWICK, FS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6068-6072
[7]   IMMUNOCHEMICAL IDENTIFICATION AND BIOLOGICAL CHARACTERIZATION OF CYTO-TOXIC NECROTIZING FACTOR FROM ESCHERICHIA-COLI [J].
DERYCKE, J ;
PHANTHANH, L ;
BERNARD, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (05) :983-988
[8]   MOLECULAR-CLONING, SEQUENCING, AND IDENTIFICATION OF A METALLOPROTEASE GENE FROM LISTERIA-MONOCYTOGENES THAT IS SPECIES-SPECIFIC AND PHYSICALLY LINKED TO THE LISTERIOLYSIN GENE [J].
DOMANN, E ;
LEIMEISTERWACHTER, M ;
GOEBEL, W ;
CHAKRABORTY, T .
INFECTION AND IMMUNITY, 1991, 59 (01) :65-72
[9]  
FUZI M, 1962, NATURE, V13, P195
[10]   INVITRO MODEL OF PENETRATION AND INTRACELLULAR GROWTH OF LISTERIA-MONOCYTOGENES IN THE HUMAN ENTEROCYTE-LIKE CELL-LINE CACO-2 [J].
GAILLARD, JL ;
BERCHE, P ;
MOUNIER, J ;
RICHARD, S ;
SANSONETTI, P .
INFECTION AND IMMUNITY, 1987, 55 (11) :2822-2829
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