A SIMPLE PROCEDURE FOR RECOVERING THE DENATURING EFFECT OF METHYLMERCURY IN AGAROSE-GEL ELECTROPHORESIS

被引：0
作者：
FERNANDEZSILVA, P [1 ]
ENRIQUEZ, JA [1 ]
MONTOYA, J [1 ]
机构：
[1] FAC VET ZARAGOZA,DEPT BIOQUIM & BIOL MOLEC & CELULAR,MIGUEL SERVET 177,E-50013 ZARAGOZA,SPAIN
来源：
BIOTECHNIQUES | 1992年 / 12卷 / 04期
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D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite(R), a mixture of anion- and cation-exchange resins. This treatment greatly improved the resolution of RNA species when fractionated by electrophoresis through agarose-CH3HgOH slab gel.
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页码：480 / &
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