BIOCHEMICAL AND FUNCTIONAL-CHARACTERIZATION OF RAPANA-THOMASIANA HEMOCYANIN

被引：35
作者：
BOTEVA, R
SEVEROV, S
GENOV, N
BELTRAMINI, M
FILIPII, B
RICCHELLI, F
TALLANDINI, L
PALLHUBER, MM
TOGNON, G
SALVATO, B
机构：
[1] CNR, CTR PHYSIOL & BIOCHEM HEMOCYANINS & OTHER MET PROT, I-35131 PADUA, ITALY
[2] UNIV PADUA, DEPT BIOL, I-35131 PADUA, ITALY
[3] UNIV PADUA, DEPT ORGAN CHEM, I-35131 PADUA, ITALY
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1991年 / 100卷 / 03期
关键词：
D O I：
10.1016/0305-0491(91)90210-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. The hemocyanin (Hc) of the marine gastropod mollusc Rapana thomasiana was collected from animals living on the west coast of the Black Sea and characterized for its biochemical and functional properties. 2. This Hc is very similar to other gastropod Hcs as far as amino acid composition, general structure and reactivity of the binuclear copper active site are concerned. 3. Some peculiarities in the dissociation-reassociation pattern are observed in comparison to other gastropod Hcs, in particular with respect to the ability to form sopramolecular aggregates. 4. Changes in pH disclose a strong reverse Bohr effect. Different R and T states are required to describe the oxygen binding curves at the different pHs.
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页码：493 / 501
页数：9
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