CONTRIBUTION OF THE B-OLIGOMER TO THE PROTECTIVE ACTIVITY OF GENETICALLY ATTENUATED PERTUSSIS TOXIN

被引：11

作者：

ARCINIEGA, JL ^{[1
]}

SHAHIN, RD ^{[1
]}

BURNETTE, WN ^{[1
]}

BARTLEY, TD ^{[1
]}

WHITELEY, DW ^{[1
]}

MAR, VL ^{[1
]}

BURNS, DL ^{[1
]}

机构：

[1] AMGEN INC,THOUSAND OAKS,CA 91320

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 10期

关键词：

D O I：

10.1128/IAI.59.10.3407-3410.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An enzymatically deficient recombinant S1 subunit, in which Arg-9 was replaced by Lys, was combined with native B oligomer to form a mutant holotoxin molecule. This molecule exhibited decreased leukocytosis-promoting and histamine-sensitizing activities compared with those of the native toxin, supporting the view that the B oligomer is not responsible for these activities. The protective activity of this genetically attenuated pertussis toxin was compared with that of B oligomer alone. The mutant pertussis toxin and B oligomer were similarly capable of protecting mice against a respiratory infection with Bordetella pertussis, suggesting that the B oligomer makes a significant contribution to the protection afforded by the genetically attenuated holotoxin.

引用

页码：3407 / 3410

页数：4

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