THE EXTENSIN SIGNAL PEPTIDE ALLOWS SECRETION OF A HETEROLOGOUS PROTEIN FROM PROTOPLASTS

被引：52

作者：

DELOOSE, M ^{[1
]}

GHEYSEN, G ^{[1
]}

TIRE, C ^{[1
]}

GIELEN, J ^{[1
]}

VILLARROEL, R ^{[1
]}

GENETELLO, C ^{[1
]}

VANMONTAGU, M ^{[1
]}

DEPICKER, A ^{[1
]}

INZE, D ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,KL LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

来源：

GENE | 1991年 / 99卷 / 01期

关键词：

CELL WALL PROTEIN; NICOTIANA-PLUMBAGINIFOLIA; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(91)90038-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Extensins are hydroxyproline-rich glycoproteins which are amongst the most abundant proteins present in the cell wall of higher plants. Here, we describe the structural analysis of an extensin-encoding gene from Nicotiana plumbaginifolia. The encoded protein (46 kDa) has a highly repetitive structure and contains 37% proline, 18.1% tyrosine, 13.4% lysine, 8.1% serine and 7.1% histidine. The extensin-encoding sequence contains a typical signal peptide for translocation of the protein to the endoplasmic reticulum. By using chimeric genes consisting of different 5' parts of the extensin-encoding gene and the neomycin phosphotransferase II-encoding gene (nptII) as reporter gene, we show that the N-terminal part of extensin can mediate the secretion of NPTII from electroporated N. tabacum protoplasts.

引用

页码：95 / 100

页数：6

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