DIACYLGLYCEROL FORMATION AND DNA-SYNTHESIS IN ENDOTHELIN-STIMULATED RAT C6 GLIOMA-CELLS - THE POSSIBLE ROLE OF PHOSPHATIDYLCHOLINE BREAKDOWN

被引：16

作者：

ZHANG, W ^{[1
]}

SAKAI, N ^{[1
]}

FU, T ^{[1
]}

OKANO, Y ^{[1
]}

HIRAYAMA, H ^{[1
]}

TAKENAKA, K ^{[1
]}

YAMADA, H ^{[1
]}

NOZAWA, Y ^{[1
]}

机构：

[1] GIFU UNIV, SCH MED, DEPT BIOCHEM, GIFU 500, JAPAN

来源：

NEUROSCIENCE LETTERS | 1991年 / 123卷 / 02期

关键词：

GLIOMA CELL; ENDOTHELIN-1; DIACYLGLYCEROL; PHOSPHATIDYLCHOLINE; DNA SYNTHESIS; PROTEIN KINASE-C;

D O I：

10.1016/0304-3940(91)90921-F

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Stimulation of C6 cells with endothelin-1 (ET) caused a biphasic sn-1,2-diacylglycerol (1,2-DG) generation, which occurred not only via phosphatidylinositol 4,5-P2 (PIP2) hydrolysis by specific phospholipase C action, but also through the breaddown of phosphatidylcholine (PC) induced by either PC phospholipase C or D. ET also stimulated DNA synthesis in serum-starved C6 cells. However, in the presence of 1-(5-isoquinolynylsulfonyl)-2-methylpiperazine (H-7), a protein kinase C (PKC) inhibitor, the [H-3]thymidine incorporation was markedly inhibited, which was concurrent with the 1,2-DG formation: the second peak was reduced. These findings suggest that brain glial cells might be an important target for ET. In addition to other possible roles, ET may also mediate mitogenesis in the brain, presumably through a PKC-dependent activation of phospholipase C and/or D hydrolyzing PC.

引用

页码：164 / 166

页数：3

共 20 条

[1] DIFFERENTIATED RAT GLIAL CELL STRAIN IN TISSUE CULTURE [J].