CELL-WALL OLIGOGALACTURONIDES INCREASE CYTOSOLIC FREE CALCIUM IN CARROT PROTOPLASTS

The mode of action of cell wall elicitors in the induction of various plant cell responses, such as the activation of host defence mechanisms, is unknown, but signal transduction through cytosolic free calcium ([Ca2+]i) has been suggested. This paper shows that polygalacturonic acid or oligogalacturonides cause a prolonged increase in [Ca2+]i of carrot protoplasts within 20 min of induction. Our data support the view that a special conformation of the oligogalacturonides possessing >9 residues is necessary to induce an elevation in [Ca2+]i. The localization of [Ca2+]i elevation around the periphery of protoplast cytoplasm and the inhibition of the response with verapamil suggest that exogenous Ca2+ is the major source for the rise in [Ca2+]i.