POSSIBLE INVOLVEMENT OF A CHLORIDE CONDUCTANCE IN THE TRANSIENT OUTWARD CURRENT OF WHOLE-CELL VOLTAGE-CLAMPED FERRET VENTRICULAR MYOCYTES

被引：11

作者：

BOURON, A ^{[1
]}

POTREAU, D ^{[1
]}

RAYMOND, G ^{[1
]}

机构：

[1] UNIV POITIERS,GEN PHYSIOL LAB,CNRS,URA 290,40 AVE RECTEUR PINEAU,F-86022 POITIERS,FRANCE

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1991年 / 419卷 / 05期

关键词：

CHLORIDE CONDUCTANCE; TRANSIENT OUTWARD CURRENT; CARDIAC CELLS; WHOLE CELL PATCH CLAMP;

D O I：

10.1007/BF00370801

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The transient outward current was studied, using the whole-cell patch-clamp technique, in isolated ventricular cells from the ferret heart. In the presence of 4-aminopyridine and cadmium chloride which respectively blocked the Ca-insensitive and the Ca-dependent outward currents, a residual transient outward current was observed in about 30% of the cells tested. This current was suppressed in external hypochloride solution, completely inhibited by SITS (3mM) and reversed at the equilibrium potential for chloride ions. This suggests the presence of a chloride permeability which could contribute to the repolarization phase of the cardiac action potential.

引用

页码：534 / 536

页数：3

共 25 条

[1] CHLORIDE CONDUCTANCE REGULATED BY CYCLIC AMP-DEPENDENT PROTEIN-KINASE IN CARDIAC MYOCYTES [J].

BAHINSKI, A ;

NAIRN, AC ;

GREENGARD, P ;

GADSBY, DC .

NATURE, 1989, 340 (6236) :718-721

[2] AN EFFICIENT ISOLATION PROCEDURE OF CA-TOLERANT VENTRICULAR MYOCYTES FROM FERRET HEART FOR APPLICATIONS IN ELECTROPHYSIOLOGICAL STUDIES [J].

BOURON, A ;

POTREAU, D ;

BESSE, C ;

RAYMOND, G .

BIOLOGY OF THE CELL, 1990, 70 (03) :121-127

[3] PROPERTIES OF THE TRANSIENT OUTWARD CURRENT IN RABBIT ATRIAL CELLS [J].

CLARK, RB ;

GILES, WR ;

IMAIZUMI, Y .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 405 :147-168

[4] EXISTENCE OF 2 TRANSIENT OUTWARD CURRENTS IN SHEEP CARDIAC PURKINJE-FIBERS [J].

CORABOEUF, E ;

CARMELIET, E .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1982, 392 (04) :352-359

[5] DETECTION OF K+ AND CL- CHANNELS FROM CALF CARDIAC SARCOLEMMA IN PLANAR LIPID BILAYER-MEMBRANES [J].

CORONADO, R ;

LATORRE, R .

NATURE, 1982, 298 (5877) :849-852

[6] SINGLE CHLORIDE-PERMEABLE CHANNELS OF LARGE CONDUCTANCE IN CULTURED CARDIAC-CELLS OF NEWBORN RATS [J].

COULOMBE, A ;

DUCLOHIER, H ;

CORABOEUF, E ;

TOUZET, N .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1987, 14 (03) :155-162

[7] IONIC CURRENTS IN CARDIAC EXCITATION [J].

DECK, KA ;

TRAUTWEIN, W .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1964, 280 (01) :63-&

[8]

DUDEL JK, 1968, PFLUGERS ARCH, V295, P197

[9] AGE-RELATED-CHANGES OF ACTION-POTENTIAL PLATEAU SHAPE IN ISOLATED HUMAN ATRIAL FIBERS [J].

ESCANDE, D ;

LOISANCE, D ;

PLANCHE, C ;

CORABOEUF, E .

AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 249 (04) :H843-H850

[10] POSITIVE DYNAMIC CURRENT AND ITS INACTIVATION PROPERTIES IN CARDIAC PURKINJE-FIBERS [J].

FOZZARD, HA ;

HIRAOKA, M .

JOURNAL OF PHYSIOLOGY-LONDON, 1973, 234 (03) :569-586

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