HPLC determination of Ursolic acid in flowers of an Iranian Pomegranate (Punica granatum L.) Cultivar

被引:0
|
作者
Fatemeh, Sharifiyan [1 ]
Abbas, Mirjalili Seyed [2 ]
Mohammad, Fazilati [1 ]
Saeed, Habibollahi [3 ]
Elahe, Poorazizi [4 ]
机构
[1] PNU, Dept Biol, Tehran, Iran
[2] AREEO, Imam Khomeini Higher Educ Ctr, Tehran, Iran
[3] PNU, Dept Chem, Tehran, Iran
[4] Islamic Azad Univ, Najafabad Branch, Esfahan, Iran
关键词
HPLC; Pomegranate flowers; Ursolic acid;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Ursolic acid (UA), is a major bioactive compound in several traditional medicinal plants including pomegranate (Punica granatum L.) flower. This study presents the HPLC analysis of UA content in flowers of an Iranian pomegranate cultivar. The analytical method's accuracy and the repeatability were satisfactory. The Linear ranges were 100-400 mu g/ml for UA with a good correlation. On the average, the recovery rate of UA was 100%. Here, the extraction of UA from an Iranian pomegranate flower cultivar by employing an ultrasound-assisted extraction method was reported, and several extraction solvents were studied to improve extraction yield of UA. Remarkable quantities of UA were found in cultivar of pomegranate flower evaluated. Our results showed that Iranian pomegranate flower cv. Ghorj Tafti Torsh is a good source for extraction and isolation of UA.
引用
收藏
页码:177 / 181
页数:5
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