ANALYSIS OF UNSTABLE RNA TRANSCRIPTS OF INSECTICIDAL CRYSTAL PROTEIN GENES OF BACILLUS-THURINGIENSIS IN TRANSGENIC PLANTS AND ELECTROPORATED PROTOPLASTS

被引：121

作者：

MURRAY, EE

ROCHELEAU, T

EBERLE, M

STOCK, C

SEKAR, V

ADANG, M

机构：

[1] UNIV WISCONSIN, DEPT ENTOMOL, MADISON, WI 53705 USA

[2] UNIV WISCONSIN, CTR BIOTECHNOL, MADISON, WI 53705 USA

[3] UNIV WISCONSIN, MCARDLE LAB CANC RES, MADISON, WI 53705 USA

[4] BLOOD CTR SE WISCONSIN INC, MILWAUKEE, WI 53233 USA

[5] MADURAI KAMARAJ UNIV, DEPT BIOTECHNOL, MADURAI 625021, TAMIL NADU, INDIA

[6] UNIV GEORGIA, DEPT ENTOMOL, ATHENS, GA 30605 USA

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 16卷 / 06期

关键词：

BACILLUS-THURINGIENSIS; CRY GENES; INSECTICIDAL CRYSTAL PROTEIN; PROTOPLAST ELECTROPORATION; RNA STABILITY; TRANSGENIC TOBACCO;

D O I：

10.1007/BF00016075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined expression of several insecticidal crystal protein (ICP) genes of Bacillus thuringiensis in transgenic tobacco plants and electroporated carrot protoplasts. We determined that low levels of lepidopteran toxin cryIA(b) ICP gene expression in plants and electroporated carrot cells is due to RNA instability. We used a series of 3' deleted cryIA(b) constructs directed by the cauliflower mosaic virus 35S promoter to demonstrate that this instability is minimally contained in the first 579 bases of the gene in both systems. This instability may result from 5' --> 3' as well as 3' --> 5' RNA metabolism. The coleopteran toxic cryIIIA gene was also examined in electroporated carrot cells, and found to be poorly expressed. A model for improvement of ICP RNA stability in plants is presented.

引用

页码：1035 / 1050

页数：16