DEMONSTRATION OF PLATELET-ACTIVATING-FACTOR RECEPTOR IN GUINEA-PIG KIDNEY

被引：19
|
作者：
TAKANO, T
HONDA, Z
WATANABE, T
UCHIDA, S
SHIMIZU, T
KUROKAWA, K
机构：
[1] UNIV TOKYO, FAC MED, DEPT INTERNAL MED 1, BUNKYO KU, TOKYO 113, JAPAN
[2] UNIV TOKYO, FAC MED, DEPT INTERNAL MED 4, BUNKYO KU, TOKYO 113, JAPAN
[3] UNIV TOKYO, DIV MED, DEPT PHYSIOL CHEM & NUTR, BUNKYO KU, TOKYO 113, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 01期
关键词：
D O I：
10.1016/0006-291X(91)91947-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Distribution of platelet activating factor (PAF) receptor was examined in the guinea pig kidney. Northern blot analysis showed a single band electrophoresed just below the 28S rRNA, and the mRNA was richest in the cortex with lesser amounts in the outer and then inner medulla. Scatchard analysis of membrane fraction using [3H]WEB 2086, a specific PAF receptor antagonist, revealed a single binding site with Bmax of 522, 228, 58 fmol/mg protein for the cortex, outer medulla and inner medulla, respectively. Kd values were in the same order of magnitude (10-8 M). These results indicate the presence of a single class of PAF receptor in the guinea pig kidney which is most abundant in the cortex. © 1991.
引用
收藏
页码：54 / 60
页数：7
相关论文
共 50 条
  • [11] INVITRO CONTRACTILE EFFECT OF PLATELET-ACTIVATING-FACTOR ON GUINEA-PIG MYOMETRIUM
    MONTRUCCHIO, G
    ALLOATTI, G
    TETTA, C
    ROFFINELLO, C
    EMANUELLI, G
    CAMUSSI, G
    PROSTAGLANDINS, 1986, 32 (04): : 539 - 554
  • [12] EFFECTS OF PLATELET-ACTIVATING-FACTOR ON LUNG EPITHELIAL PERMEABILITY IN THE GUINEA-PIG
    MACQUINMAVIER, I
    FRANCOMONTOYA, ML
    ROSSO, J
    JARREAU, PH
    MEIGNAN, M
    HARF, A
    PULMONARY PHARMACOLOGY, 1991, 4 (04): : 233 - 238
  • [13] RECRUITMENT OF EOSINOPHILS BY PLATELET-ACTIVATING-FACTOR (PAF) IN THE GUINEA-PIG LUNG
    AOKI, S
    BOUBEKEUR, K
    BURROWS, L
    MORLEY, J
    SANJAR, S
    JOURNAL OF PHYSIOLOGY-LONDON, 1987, 394 : P130 - P130
  • [14] PLATELET-ACTIVATING-FACTOR STIMULATES A RECEPTOR-COUPLED MEMBRANE GTPASE IN GUINEA-PIG EOSINOPHILS
    DENT, G
    BARNES, PJ
    LIFE SCIENCES, 1993, 52 (20) : 1633 - 1640
  • [15] EXPRESSION OF PLATELET-ACTIVATING-FACTOR RECEPTOR MESSENGER-RNA IN HUMAN AND GUINEA-PIG LUNG
    SHIRASAKI, H
    NISHIKAWA, M
    ADCOCK, IM
    MAK, JCW
    SAKAMOTO, T
    SHIMIZU, T
    BARNES, PJ
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1994, 10 (05) : 533 - 537
  • [16] THE ANAPHYLACTIC RELEASE OF PLATELET-ACTIVATING-FACTOR FROM PERFUSED GUINEA-PIG LUNGS
    FITZGERALD, MF
    MONCADA, S
    PARENTE, L
    BRITISH JOURNAL OF PHARMACOLOGY, 1986, 88 (01) : 149 - 153
  • [17] STUDY OF CARDIOVASCULAR EFFECTS OF THE PLATELET-ACTIVATING-FACTOR (PAF) IN THE RAT AND THE GUINEA-PIG
    TANNIERE, M
    ROCHETTE, L
    ARCHIVES DES MALADIES DU COEUR ET DES VAISSEAUX, 1986, 79 (04): : 576 - 576
  • [18] BIOLOGICAL RESPONSE OF GUINEA-PIG PERITONEAL-MACROPHAGES TO PLATELET-ACTIVATING-FACTOR
    HAYASHI, H
    KUDO, I
    NOJIMA, S
    INOUE, K
    LIPIDS, 1991, 26 (12) : 1193 - 1199
  • [19] PLATELET-ACTIVATING-FACTOR INCREASES VASCULAR-PERMEABILITY IN GUINEA-PIG AIRWAYS
    EVANS, TW
    CHUNG, KF
    BARNES, PJ
    CLINICAL SCIENCE, 1986, 71 : P1 - P1
  • [20] THE EFFECTS OF PLATELET-ACTIVATING-FACTOR ON THE OUTPUT OF PROSTAGLANDINS FROM THE GUINEA-PIG UTERUS
    NORMAN, SJ
    POYSER, NL
    PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1992, 47 (04): : 285 - 289
12345