EFFECT OF VITAMIN-A ACID ON GROWTH OF KERATINOCYTES IN CULTURE

被引：13
作者：
WILKINSON, DI
机构：
[1] Department of Dermatology, Stanford University, School of Medicine, Stanford, 94305, California
来源：
ARCHIVES OF DERMATOLOGICAL RESEARCH | 1978年 / 263卷 / 01期
关键词：
DNA levels; Keratinocyte cell culture; Retinoic acid; Thymidine incorporation;
D O I：
10.1007/BF00446856
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incoporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24-45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes. © 1978 Springer-Verlag.
引用
收藏
页码：75 / 81
页数：7
相关论文
共 18 条
12