This manuscript reports the results of our study to assess the influence of stress due to job, strategies adopted for coping the stress, their association, and effect on performance and psychological well-being of agricultural research sector (ARS) employees. A survey of 700 staff working in the ARS in Hyderabad Metro, consisting of 360 women and 340 men were carried out. The 14 independent job stress components, harassment, role ambiguity, psychological factors, peer support, workload, co-workers, role conflict, career, physiological factors, behavioral factors, job control and social support, strategies of coping both the approach & avoidance strategies on dependent factors Performance and Psychological well-being of the agricultural research sector employees were estimated. The Cronbach's alpha value for the whole sample is 0.87, and C-alpha values for the job stress components and outcome factors performance and psychological well-being ranged between 0.70-0.81. The odds ratios (ORs) were measured to calculate the level of relation of job stress, coping methods, relationship with performance and psychological well-being of ARS staff. The age and gender differences were also studied.