MODEL FOR PREDICTING THE PARTITION BEHAVIOR OF PROTEINS IN AQUEOUS 2-PHASE SYSTEMS

被引：81

作者：

ASENJO, JA

SCHMIDT, AS

HACHEM, F

ANDREWS, BA

机构：

[1] Biochemical Engineering Laboratory, University of Reading, Reading

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 668卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80090-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of protein hydrophobicity, charge, molecular mass and concentration has been studied in poly(ethylene glycol) (PEG)-phosphate and PEG-dextran aqueous two-phase systems in the presence and absence of NaCl for several model proteins. The surface hydrophobicity of the protein measured by precipitation correlated well with the partition coefficient in PEG-salt systems at high levels of NaCl. The charge of proteins also has an important effect on partition; this is expected to be more pronounced at lower NaCl concentrations. For molecular mass a tendency was found in PEG-dextran systems at low NaCl concentrations. No clear tendency was observed in the PEG-salt systems. The solubility of the protein in the phases also affects its partition behaviour. This behaviour was fitted to a saturation type equation for alpha-amylase in each of the phases of a PEG-phosphate system

引用

页码：47 / 54

页数：8

共 15 条

[1]

Abbott N L, 1990, Bioseparation, V1, P191

[2]

ASENJO JA, 1991, BIOLOGICALS FROM RECOMBINANT MICROORGANISMS AND ANIMAL CELLS, P439

[3] PROTEIN PARTITIONING IN 2-PHASE AQUEOUS POLYMER SYSTEMS [J].