CCAAT/ENHANCER BINDING-PROTEINS ALPHA AND BETA ARE TRANSCRIPTIONAL ACTIVATORS OF THE BROWN FAT UNCOUPLING PROTEIN GENE PROMOTER

被引：63

作者：

YUBERO, P ^{[1
]}

MANCHADO, C ^{[1
]}

CASSARDDOULCIER, AM ^{[1
]}

MAMPEL, T ^{[1
]}

VINAS, O ^{[1
]}

IGLESIAS, R ^{[1
]}

GIRALT, M ^{[1
]}

VILLARROYA, F ^{[1
]}

机构：

[1] CTR RECH ENDOCRINOL MOLEC & DEV,CNRS,MEUDON,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary brown adipocytes differentiated in culture were transiently transfected with plasmids containing different extensions of the 5′-flanking region of the rat uncoupling protein gene placed upstream of the bacterial chloramphenicol acetyltransferase reporter gene. Co-transfection of expression vectors for CCAAT/enhancer binding protein (C/EBP)α and C/EBPβ trans-activated the rat uncoupling protein gene promoter due to sequences in the 5′ proximal region. DNASe I footprint analysis showed the presence of two C/EBP binding sites at positions -457/-440 and -335/-318, which interact with purified C/EBPβ as well as with C/EBP proteins present in brown fat or liver nuclear extracts. Two copies of each site placed upstream of the enhancerless SV40 promoter confer C/EBPα and C/EBPβ responsiveness to this heterologous promoter when co-transfected into HepG2 cells. It is concluded that the UCP gene is a target for C/EBP-dependent transcriptional regulation. This suggests that the C/EBP family of transcription factors is involved in the establishment of the characteristic phenotype of the brown adipocyte. © 1994 Academic Press, Inc.

引用

页码：653 / 659

页数：7

共 28 条

[1] A NUCLEAR FACTOR FOR IL-6 EXPRESSION (NF-IL6) IS A MEMBER OF A C/EBP FAMILY [J].