A HIGH-AFFINITY PROTEIN STAIN FOR WESTERN BLOTS, TISSUE PRINTS, AND ELECTROPHORETIC GELS

被引：51

作者：

BICKAR, D ^{[1
]}

REID, PD ^{[1
]}

机构：

[1] SMITH COLL,DEPT BIOL SCI,NORTHAMPTON,MA 01063

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 203卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90049-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for protein staining using copper phthalocyanine 3,4′,4″,4‴-tetrasulfonic acid tetrasodium salt is described. The procedure is applicable to protein blots and tissue prints, as well as to polyacrylamide and agarose gels. It is also simple, involving only application of the stain and rinsing. For protein blots and tissue prints the staining is rapid, taking less than 1 min to completion, and more sensitive than any previously described dye-based nonspecific protein staining system. The staining is easily reversible, requiring only a change in pH to remove the dye. © 1992.

引用

页码：109 / 115

页数：7

共 23 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].