COUPLING SITE-I AND THE ROTENONE-SENSITIVE UBISEMIQUINONE IN TIGHTLY COUPLED SUBMITOCHONDRIAL PARTICLES

被引：78
作者：
KOTLYAR, AB
SLED, VD
BURBAEV, DS
MOROZ, IA
VINOGRADOV, AD
机构：
[1] MV LOMONOSOV STATE UNIV, SCH BIOL, DEPT BIOCHEM, MOSCOW 119899, USSR
[2] ACAD SCI USSR, INST CHEM PHYS, MOSCOW 117977, USSR
来源：
FEBS LETTERS | 1990年 / 264卷 / 01期
关键词：
Electron transfer; Mitochrondria; NADH-ubiquinone reductase; Ubisemiquinone;
D O I：
10.1016/0014-5793(90)80753-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The rotenone-sensitive g = 2.00 low temperature EPR signal attributed to ubisemiquinone is observed in submitochondrial particles during coupled electron transfer from NADH to oxygen and from succinate to NAD+. The signal is seen only in the presence of oligomycin added to induce the respiratory control (7-9 with NADH and 3-4 with succinate) and it disappears in the presence of uncouplers (CCCP or gramicidin D). No reduction of the iron-sulfur center N-2 in the presence of 20 mM succinate and cyanide is observed, thus suggesting that N-2 is not in equilibrium with the ubiquinone pool. A hypothesis is proposed on 7Delta;smH+ generation coupled with electron transfer between iron-sulfur center N-2 and the ubiquinone pool. © 1990.
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页码：17 / 20
页数：4
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