CYTOTOXICITY TO CULTURED HUMAN KERATINOCYTES OF TOPICAL ANTIMICROBIAL AGENTS

被引:71
作者
COOPER, ML
BOYCE, ST
HANSBROUGH, JF
FOREMAN, TJ
FRANK, DH
机构
[1] Department of Surgery, University of California, San Diego Medical Center, San Diego
关键词
D O I
10.1016/0022-4804(90)90212-K
中图分类号
R61 [外科手术学];
学科分类号
摘要
Cultured skin grafts administered clinically for closure of burn wounds may be contacted by topically applied antimicrobial agents. A study was performed to assess whether commonly used topical antimicrobial agents are toxic to cultured human keratinocytes (HK) in vitro. Serum-free MCDB 153 culture medium containing Neosporin G.U. irrigant (Neomycin, 40 μg/ml-polymyxin B sulfate, 200 units/ml) and a standard tissue culture antimicrobial agent of penicillin (10,000 units/ml)-streptomycin (10,000 μg/ml)-amphotericin B (25 μg/ml) had no effect on the keratinocyte growth rates when compared to standard MCDB 153 medium without antibiotics. Medium containing Sulfamylon (mafenide acetate, 0.85%), Polysporin (polymyxin B sulfate, 1 × 104 units/ml-bacitracin, 500 units/ml), gentamicin sulfate (0.1%), modified Dakins solution (25%), and acetic acid (0.25%) all showed statistically significant (P < 0.01) decreases in keratinocyte growth rates. This data suggests that commonly applied antimicrobials may not be appropriate for cultured grafts in the concentrations that are used clinically. © 1990.
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页码:190 / 195
页数:6
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