Purification and characterization of extracellular phospholipase A(1) from Trichoderma atroviride sp. ZB-ZH292

被引：3

作者：

Beig-Mohammadi, Zahra ^{[1
]}

Hamidi-Esfahani, Zohreh ^{[1
]}

Sahari, Mohammad-Ali ^{[1
]}

Khosravi-Darani, Kianoush ^{[2
]}

机构：

[1] Tarbiat Modares Univ, Fac Agr, Dept Food Sci & Technol, POB 14115-336, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Fac Nutr Sci & Food Technol, Tehran, Iran

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2018年 / 13卷

关键词：

Phospholipase A(1); Trichoderma atroviride; Purification; Characterization; Zymography;

D O I：

10.1016/j.bcab.2017.11.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phospholipase A(1) has been used in degumming process of vegetable oils and release functional free fatty acids and lysophospholipid. This study was aimed to isolate and identify a new Trichoderma atroviride sp. ZB-ZH192 as producer of PLA(1). Then gamma rays mutants with increased efficiency of extracellular production were used for purification and characterization of PLA(1). The maximum protein content (0.03 mg), activity (594 U), and specific activity (1220 U/mg protein) of enzyme from T. atroviride sp. ZB-ZH292 was found at pH 6 and 60 degrees C, which is more comparing to wild type. The molecular weight of phospholipase A1 was detected as 40 kDa by electrophoresis. To confirm the activity of phospholipase, zymography method was used. Results indicate that the purified phospholipase A(1) from mutant source had suitable potential to serve as a biocatalyst in vegetable oil processing degumming.

引用

页码：176 / 181

页数：6

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