DETERMINATION OF COCAINE AND BENZOYLECGONINE BY DERIVATIZATION WITH IODOMETHANE-D(3) OR PFPA/HFIP IN HUMAN BLOOD AND URINE USING GC/MS (EI OR PCI MODE)

被引:20
作者
ADERJAN, RE
SCHMITT, G
WU, M
MEYER, C
机构
[1] Institute for Forensic Medicine, University of Heidelberg, Heidelberg, D-6900
关键词
D O I
10.1093/jat/17.1.51
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for the simultaneous detection and quantification of cocaine and benzoylecgonine from human blood and urine has been developed. Cocaine and benzoylecgonine were extracted from blood and urine with solid-phase columns containing C18 packing material. Clean, emulsion-free extracts were obtained. The analytes were derivatized with PFPA/HFIP or iodomethane-D3. The underivatized cocaine and derivatized benzoylecgonine were determined by GC/MS with positive chemical ionization (PCI) and the selected ion monitoring mode. Benzoylecgonine was derivatized with iodomethane-D3 to cocaine-D3. Both derivatives were chromatographed on a CP-SIL 5 capillary column. The recovery of extraction was greater than 70%. The detection limit for the PFPA/HFIP derivatives (20 ng/mL) was better than that for iodomethane-D3 derivatives (40 ng/mL). The procedure is simple, rapid, and reproducible. Standard curves were linear and the procedure was suitable for routine analysis. Using fluorescence polarization Immunoassay (TDx, Abbott) (cutoff 100 ng/mL), along with one of the GC/MS methods described, we found no false positive or false negative results. © 1993 Oxford University Press.
引用
收藏
页码:51 / 55
页数:5
相关论文
共 17 条
[1]   THE URINARY-EXCRETION OF COCAINE AND METABOLITES IN HUMANS - A KINETIC-ANALYSIS OF PUBLISHED DATA [J].
AMBRE, J .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1985, 9 (06) :241-245
[2]   THE DETERMINATION OF DRUGS OF ABUSE IN WHOLE-BLOOD BY MEANS OF FPIA AND EMIT-DAU IMMUNOASSAYS - A COMPARATIVE-STUDY [J].
BOGUSZ, M ;
ADERJAN, R ;
SCHMITT, G ;
NADLER, E ;
NEUREITHER, B .
FORENSIC SCIENCE INTERNATIONAL, 1990, 48 (01) :27-37
[3]   GAS CHROMATOGRAPHY-CHEMICAL IONIZATION MASS-SPECTROMETRY OF COCAINE AND ITS METABOLITES IN BIOLOGICAL-FLUIDS [J].
CHINN, DM ;
CROUCH, DJ ;
PEAT, MA ;
FINKLE, BS ;
JENNISON, TA .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1980, 4 (01) :37-42
[4]   DETECTION OF O-6-MONOACETYLMORPHINE IN URINE SAMPLES BY GC/MS AS EVIDENCE FOR HEROIN USE [J].
FEHN, J ;
MEGGES, G .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1985, 9 (03) :134-138
[5]   GC-MS DETERMINATION OF BENZOYLECGONINE IN URINE FOLLOWING AN EXTRACTIVE ALKYLATION TECHNIQUE [J].
GRAAS, JE ;
WATSON, E .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1978, 2 (03) :80-82
[6]  
HO YS, 1990, J FORENSIC SCI, V35, P123
[7]   A METHOD FOR THE SIMULTANEOUS DETERMINATION OF COCAINE, BENZOYLECGONINE, AND ECGONINE METHYL-ESTER IN BLOOD AND URINE USING GC EIMS WITH DERIVATIZATION TO PRODUCE HIGH MASS MOLECULAR-IONS [J].
ISENSCHMID, DS ;
LEVINE, BS ;
CAPLAN, YH .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1988, 12 (05) :242-245
[8]   MASS-SPECTROMETRIC DETERMINATION OF COCAINE AND ITS BIOLOGICALLY-ACTIVE METABOLITE, NORCOCAINE, IN HUMAN-URINE [J].
JINDAL, SP ;
LUTZ, T ;
VESTERGAARD, P .
BIOMEDICAL MASS SPECTROMETRY, 1978, 5 (12) :658-663
[9]   QUANTITATION OF COCAINE AND ITS PRINCIPAL METABOLITE, BENZOYLECGONINE, BY GLC-MASS SPECTROMETRY USING STABLE ISOTOPE LABELED ANALOGS AS INTERNAL STANDARDS [J].
JINDAL, SP ;
VESTERGAARD, P .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1978, 67 (06) :811-814
[10]  
KLEIN M, 1987, FORESNSIC MASS SPECT, P53