ANCHORAGE-INDEPENDENT GROWTH AND THE EXPRESSION OF CELLULAR PROTOONCOGENES IN NORMAL HUMAN EPIDERMAL-KERATINOCYTES AND IN HUMAN SQUAMOUS-CELL CARCINOMA CELL-LINES

被引：9
作者：
KIM, K
AKOTOAMFU, E
CHERRICK, HM
PARK, NH
机构：
[1] UNIV CALIF LOS ANGELES,SCH DENT,ORAL BIOL SECT,10833 LE CONTE AVE,LOS ANGELES,CA 90024
[2] UNIV CALIF LOS ANGELES,SCH DENT,ORAL & MAXILLOFACIAL SURG SECT,LOS ANGELES,CA 90024
[3] UNIV CALIF LOS ANGELES,DENT RES INST,LOS ANGELES,CA 90024
[4] UNIV CALIF LOS ANGELES,JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90024
来源：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS | 1991年 / 71卷 / 03期
关键词：
D O I：
10.1016/0030-4220(91)90305-V
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The expression of multiple cellular proto-oncogenes and the in vitro anchorage-independent growth of normal human epidermal keratinocytes and several human squamous cell carcinoma cell lines were studied and correlated. Squamous cell carcinoma cell lines KB, Si Ha, HEp-2, and Fa Du showed high anchorage independency, and MS 751 and A-253 cell lines had minimum independency. However, the normal keratinocytes and the A-431 cell line did not show anchorage-independent growth. Both the normal human epidermal keratinocytes and cancer cell lines expressed multiple proto-oncogenes such as src, erb B-1, abl, fos, raf, H-ras, and myc, and the amount of expression of these oncogenes was notably higher in the cancer cell lines than in the normal keratinocytes. The expression of proto-oncogenes from the monolayer cultures of the cancer cell lines is poorly correlated with the anchorage independency of the cells. These data indicate that the anchorage independency is not directly linked to the expression of specific cellular proto-oncogene(s) of the monolayer cancer cell cultures.
引用
收藏
页码：303 / 311
页数：9
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