TRANSCRIPTIONAL REGULATION OF RAT-LIVER PROTEIN DISULFIDE-ISOMERASE GENE BY INSULIN AND IN DIABETES

被引：37
作者：
NIETO, A
MIRA, E
CASTANO, JG
机构：
[1] UNIV AUTONOMA MADRID,FAC MED,DEPT BIOQUIM,E-28049 MADRID,SPAIN
[2] UNIV AUTONOMA MADRID,FAC MED,CSIC,INST INVESTIGAC BIOMED,E-28029 MADRID,SPAIN
来源：
BIOCHEMICAL JOURNAL | 1990年 / 267卷 / 02期
关键词：
D O I：
10.1042/bj2670317
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mRNA encoding for rat protein disulphide-isomerase (PDI) increases 3-fold in the liver of diabetic rats and is accompanied by similar changes at the protein level. Long treatment (for 3 days) of diabetic rats with insulin reverses this effect of diabetes both at the mRNA and protein levels. The higher expression of rat PDI mRNA in diabetes is due to an increase in the transcriptional rate of the gene, and insulin treatment of diabetic animals produces within 30 min a decrease in the level of transcription of PDI gene, as judged by nuclear run-on transcription experiments performed in vivo. These results clearly show a role for insulin in the regulation of transcription of the gene encoding this multifunctional protein in rat liver.
引用
收藏
页码：317 / 323
页数：7
相关论文
共 39 条
[1]   LEVELS OF TRANSLATABLE MESSENGER-RNAS FOR CELL-SURFACE PROTEIN, COLLAGEN PRECURSORS, AND 2 MEMBRANE PROTEINS ARE ALTERED IN ROUS-SARCOMA VIRUS-TRANSFORMED CHICK-EMBRYO FIBROBLASTS [J].
ADAMS, SL ;
SOBEL, ME ;
HOWARD, BH ;
OLDEN, K ;
YAMADA, KM ;
CROMBRUGGHE, BD ;
PASTAN, I .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3399-3403
[2]  
ANFINSEN CB, 1975, ADV PROTEIN CHEM, V29, P209
[3]   NUCLEOTIDE-SEQUENCE OF RAT-LIVER IODOTHYRONINE 5'-MONODEIODINASE (5'MD) - ITS IDENTITY WITH THE PROTEIN DISULFIDE ISOMERASE [J].
BOADO, RJ ;
CAMPBELL, DA ;
CHOPRA, IJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 155 (03) :1297-1304
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   THE BIOCHEMISTRY OF THE COMPLICATIONS OF DIABETES-MELLITUS [J].
BROWNLEE, M ;
CERAMI, A .
ANNUAL REVIEW OF BIOCHEMISTRY, 1981, 50 :385-432
[6]   DEFECTIVE CO-TRANSLATIONAL FORMATION OF DISULFIDE BONDS IN PROTEIN DISULFIDE-ISOMERASE-DEFICIENT MICROSOMES [J].
BULLEID, NJ ;
FREEDMAN, RB .
NATURE, 1988, 335 (6191) :649-651
[7]  
CHENG SY, 1987, J BIOL CHEM, V262, P11221
[8]  
CIMBALA MA, 1982, J BIOL CHEM, V257, P7629
[9]   SEQUENCE OF PROTEIN DISULFIDE ISOMERASE AND IMPLICATIONS OF ITS RELATIONSHIP TO THIOREDOXIN [J].
EDMAN, JC ;
ELLIS, L ;
BLACHER, RW ;
ROTH, RA ;
RUTTER, WJ .
NATURE, 1985, 317 (6034) :267-270
[10]  
FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266
1234