CLONING AND EXPRESSION OF THE GENE ENCODING THE 7FE TYPE FERREDOXIN FROM A THERMOPHILIC HYDROGEN-OXIDIZING BACTERIUM, BACILLUS-SCHLEGELII

被引：18

作者：

AONO, S ^{[1
]}

NAKAMURA, S ^{[1
]}

AONO, R ^{[1
]}

OKURA, I ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,MIDORI KU,YOKOHAMA 227,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1792

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural gene (fdxA) coding for a 7Fe type ferredoxin of a oxidizing bacterium Bacillus schlegelii has been cloned and thermophilic hydrogen sequenced. The fdxA coding region is 231 nucleotides which codes for a 77 amino acids protein with the molecular weight of 8,744 except for iron-sulfur dusters. The fdxA gene has been expressed in E. coli to obtain the recombinant ferredoxin. The recombinant ferredoxin have shown the identical properties to the native one for absorption spectra, and SDS-PAGE. (C) 1994 Academic Press, Inc.

引用

页码：938 / 942

页数：5

共 15 条

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