BIOLUMINESCENCE OF THE CA2+-BINDING PHOTOPROTEIN, AEQUORIN, AFTER HISTIDINE MODIFICATION

被引：48
作者：
OHMIYA, Y
TSUJI, FI
机构：
[1] OSAKA BIOSCI INST,6-2-4 FURUEDAI,SUITA,OSAKA 565,JAPAN
[2] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,DIV MARINE BIOL RES,LA JOLLA,CA 92093
来源：
FEBS LETTERS | 1993年 / 320卷 / 03期
关键词：
CALCIUM; APOAEQUORIN; COELENTERAZINE; DIETHYL PYROCARBONATE; OXYGEN BINDING;
D O I：
10.1016/0014-5793(93)80600-Y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Modification studies of the 5 histidine residues in aequorin employing site-directed mutagenesis and diethyl pyrocarbonate suggested that His169 may be the site of binding of molecular oxygen in aequorin. The modification of this residue led to complete loss of activity, whereas modification of the remaining 4 histidine residues yielded mutant aequorins with varying bioluminescence activities.
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页码：267 / 270
页数：4
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