A METHOD FOR SIMULTANEOUS DETECTION OF MULTIPLE MESSENGER-RNAS USING DIGOXIGENIN AND RADIOISOTOPIC CRNA PROBES

被引：59

作者：

MILLER, MA ^{[1
]}

KOLB, PE ^{[1
]}

RASKIND, MA ^{[1
]}

机构：

[1] VET AFFAIRS MED CTR,CTR RES EDUC & CLIN,SEATTLE,WA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1993年 / 41卷 / 12期

关键词：

IN SITU HYBRIDIZATION; MESSENGER-RNA; DIGOXIGENIN; NONISOTOPIC RIBOPROBES;

D O I：

10.1177/41.12.8245421

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We present a simple, reliable method for simultaneous detection of two distinct mRNAs within the same tissue sample by double in situ hybridization histochemistry. Sections are hybridized with a cocktail of radiolabeled and digoxigenin-labeled cRNA probes. The digoxigenin-labeled probe is detected with an alkaline phosphatase (AP)-dependent chromogen reaction and then the radiolabeled probe is detected by conventional autoradiography. The sensitivities of the two detection methods are comparable and demonstrate the feasibility of using double in situ hybridization histochemistry to investigate the co-localization and co-regulation of mRNAs that are expressed at relatively low levels.

引用

页码：1741 / 1750

页数：10