INVITRO PROCESSING OF THE RNA-2-ENCODED POLYPROTEIN OF 2 NEPOVIRUSES - TOMATO BLACK RING VIRUS AND GRAPEVINE CHROME MOSAIC-VIRUS

被引：24
作者：
DEMANGEAT, G
HEMMER, O
FRITSCH, C
LE GALL, O
CANDRESSE, T
机构：
[1] UNIV STRASBOURG 1, CNRS, INST BIOL MOLEC PLANTES, 12 RUE GEN ZIMMER, F-67084 STRASBOURG, FRANCE
[2] INRA, CTR RECH BORDEAUX, PATHOL VEGETALE STN, F-33883 VILLENAVE DORNON, FRANCE
来源：
JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷
关键词：
D O I：
10.1099/0022-1317-72-2-247
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In vitro translation of RNA-2 of each of two closely related nepoviruses, tomato black ring virus (TBRV) and grapevine chrome mosaic virus (GCMV), in a rabbit reticulocyte lysate resulted in the synthesis of single polypeptides of 150K and 146K respectively. Processing of these polyproteins occurred after the addition of translation products of homologous RNA-1. The positions of the cleavage products within the polyproteins were determined. From the N to the C terminus, M(r) values for the proteins were 50K, 46K and 59K for TBRV and 44K, 46K and 56K for GCMV. TBRV RNA-1 translation products also cleaved the polyproteins encoded by GCMV RNA-2 which suggests that the cleavage sites in the two polyproteins are similar.
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页码：247 / 252
页数：6
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