MOLECULAR ANALYSIS OF COMPLEX HUMAN CELL-POPULATIONS - MUTATIONAL SPECTRA OF MNNG AND ICR-191

被引：77

作者：

CARIELLO, NF ^{[1
]}

KEOHAVONG, P ^{[1
]}

KAT, AG ^{[1
]}

THILLY, WG ^{[1
]}

机构：

[1] MIT,CTR ENVIRONM HLTH SCI,CAMBRIDGE,MA 02139

来源：

MUTATION RESEARCH | 1990年 / 231卷 / 02期

关键词：

D O I：

10.1016/0027-5107(90)90023-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe a method to identify and enumerate mutants at the nucleotide level in complex cell populations. Several thousand different mutants were induced at the HPRT locus in human lymphoblastoid cultures by either MNNG, an alkylating agent, or by ICR-191, a substituted acridine. HPRT mutants were selected en masse by resistance to 6-thioguanine. The most frequent mutations (hotspots) in HPRT exon 3 were determined by a combination of denaturing gradient gel electrophoresis and polymerase chain reaction. MNNG predominantly produced GC → AT transitions at nucleotides in a GGGGGG sequence, while ICR-191 produced both +1 frameshifts in the same GGGGGG sequence and +1 frameshifts in a CCC sequence. © 1990.

引用

页码：165 / 176

页数：12

共 56 条

[1] ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].