RECOMBINANT HUMAN-MOUSE CHIMERIC MONOCLONAL-ANTIBODY SPECIFIC FOR HUMAN ADENOCARCINOMA ASSOCIATED ANTIGEN

被引:6
|
作者
NAKASHIMA, M [1 ]
NISHIMURA, Y [1 ]
WATANABE, T [1 ]
机构
[1] KYUSHU UNIV,MED INST BIOREGULAT,DEPT MOLEC IMMUNOL,MAIDASHI 3-1-1,HIGASHI KU,FUKUOKA 812,JAPAN
来源
HYBRIDOMA | 1991年 / 10卷 / 01期
关键词
D O I
10.1089/hyb.1991.10.1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have recently described a class-switched (IgM to IgG1) human-mouse chimeric antibody. In the present study, a human-mouse chimeric antibody specific for human adenocarcinoma-associated antigen YH206 antigen was constructed by fusing murine variable region genes (V-kappa and V(H)) to human constant region (gamma-1,kappa). The murine variable domain genes were isolated from a functional murine hybridoma cell line, YH206, which secreted IgM monoclonal antibody specific for YH206 antigen. The fusion genes of heavy and light chains were introduced into the immunoglobulin non-producing mouse myeloma cell line X63-Ag8.653 by electroporation. We obtained transformants which secreted class-switched human-mouse chimeric antibodies specific for YH206 antigen. A dot immunobinding assay demonstrated that the class-switched chimeric antibody retained the ability to bind to the YH206 antigen.
引用
收藏
页码:1 / 9
页数:9
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