TRANSPOSABLE ELEMENTS AS PLANT TRANSFORMATION VECTORS FOR LONG STRETCHES OF FOREIGN DNA

被引：11

作者：

LEBEL, EG ^{[1
]}

MASSON, J ^{[1
]}

BOGUCKI, A ^{[1
]}

PASZKOWSKI, J ^{[1
]}

机构：

[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 91卷 / 6-7期

关键词：

TRANSFORMATION VECTORS; TRANSPOSITION; AC/DS; PLANTS;

D O I：

10.1007/BF00223898

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The production of transgenic plants is now routine for most crops. However, using currently available transformation methods it is still difficult and time-consuming to obtain a collection of transformed individuals containing single or low-copy-number, intact transgenic inserts. Here we describe a set of broad-host-range transformation vectors based on the Ac/Ds transposition system that improve both transformation efficiency and the quality of transgenic loci. These vectors efficiently deliver long stretches of foreign DNA into the genome, leading to transgenic strains containing an intact single-copy insert of 10kb. This type of vector could be an important additional tool for the production of transgenic plants with the well-defined, foreign DNA inserts required for biosafety approval and commercialisation.

引用

页码：899 / 906

页数：8

共 44 条

[1] TRANSPOSITION OF THE MAIZE CONTROLLING ELEMENT ACTIVATOR IN TOBACCO [J].