IN-VITRO CULTURE OF ALOE-BARBADENSIS MILL - QUANTITATIVE DNA VARIATIONS IN REGENERATED PLANTS

被引：3
作者：
CAVALLINI, A
NATALI, L
CIONINI, G
SASSOLI, O
CASTORENASANCHEZ, I
机构：
[1] IST MUTAGENESI & DIFFERENZIAMENTO, PISA, ITALY
[2] CTR INVEST CIENT YUCATAN, MERIDA, MEXICO
来源：
PLANT SCIENCE | 1993年 / 91卷 / 02期
关键词：
ALOE-BARBADENSIS MILL; BASIC DNA CONTENT VARIATION; CELL DIMENSIONS; CYTOPHOTOMETRY; HETEROCHROMATIN; REGENERATED PLANTS;
D O I：
10.1016/0168-9452(93)90145-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Six plants of Aloe barbadensis regenerated from callus were studied as far as chromosome number: five plants were diploid and one tetraploid. Cytophotometric analyses of Feulgen-stained early-prophase nuclei showed a 22.5% basic DNA content variation among diploid plants. Both increases and diminutions of DNA were recorded, related to precise chromatin fractions (either euchromatin and heterochromatin), as distinguished by their condensation in interphase nuclei. Variations were also found among regenerated plants related to the amount of ribosomal DNA. As far as the phenotypic effect of these variations, analysis of epidermal cell area showed that nuclear DNA content affects positively cell dimensions.
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页码：223 / 229
页数：7
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