CA2+ BINDING OF LATENT TRANSFORMING GROWTH-FACTOR-BETA-1 BINDING-PROTEIN

被引：22
|
作者：
COLOSETTI, P
HELLMAN, U
HELDIN, CH
MIYAZONO, K
机构：
[1] Ludwig Institute/or Cancer Research, Biomedical Center, S-751 24 Uppsala
来源：
FEBS LETTERS | 1993年 / 320卷 / 02期
关键词：
TRANSFORMING GROWTH FACTOR-BETA; BINDING PROTEIN; EGF-LIKE REPEAT; CA2+ BINDING;
D O I：
10.1016/0014-5793(93)80079-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Latent transforming growth factor-beta1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta1 complex. The binding of Ca2+ induces a structural change which protects the molecule against proteolysis and changes its elution position when analyzed by anion exchange chromatography. The in vitro activation of TGF-beta1 is not influenced by the presence of Ca2+. The possible significance of Ca2+ binding of LTBP is discussed.
引用
收藏
页码：140 / 144
页数：5
相关论文
共 50 条
  • [1] CHARACTERIZATION OF BINDING OF TRANSFORMING GROWTH-FACTOR-BETA-1 TO BOVINE MAMMARY MEMBRANES
    PLAUT, K
    MAPLE, RL
    JOURNAL OF DAIRY SCIENCE, 1995, 78 (07) : 1463 - 1469
  • [2] Immunohistochemical localization of latent transforming growth factor-beta binding protein in IgA nephropathy
    Wada, T
    Hamakawa, S
    Hori, Y
    Kaname, S
    Shimizu, S
    Kurokawa, K
    Katoh, T
    KIDNEY INTERNATIONAL, 1997, : S182 - S184
  • [3] Latent transforming growth factor-beta 1 and its binding protein are components of extracellular matrix microfibrils
    Taipale, J
    Saharinen, J
    Hedman, K
    KeskiOja, J
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1996, 44 (08) : 875 - 889
  • [4] Expression of alternatively spliced human latent transforming growth factor β binding protein-1
    Öklü, R
    Hesketh, TR
    Metcalfe, JC
    Kemp, PR
    FEBS LETTERS, 1998, 435 (2-3): : 143 - 148
  • [5] Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1
    Saharinen, J
    Taipale, J
    KeskiOja, J
    EMBO JOURNAL, 1996, 15 (02) : 245 - 253
  • [6] alpha(2)-Macroglobulin: A binding protein for transforming growth factor-beta and various cytokines
    Feige, JJ
    Negoescu, A
    Keramidas, M
    Souchelnitskiy, S
    Chambaz, EM
    HORMONE RESEARCH, 1996, 45 (3-5) : 227 - 232
  • [7] Induction and coexpression of latent transforming growth factor β-binding protein-1 and fibrillin-1 in experimental glomerulonephritis
    Porst, M
    Daniel, C
    Plank, C
    Schocklmann, HO
    Reinhardt, DP
    Hartner, A
    NEPHRON EXPERIMENTAL NEPHROLOGY, 2006, 102 (3-4): : E99 - E104
  • [8] EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA-1 ON MYOCARDIAL STUNNING IN THE INTACT DOG
    SUN, JZ
    LI, XY
    SPORN, MB
    SCHNEIDER, MD
    ROBERTS, R
    BOLLI, R
    JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1993, 25 (04) : 379 - 386
  • [9] ALTERED EXPRESSION OF TRANSFORMING GROWTH-FACTOR-BETA-1 MESSENGER-RNA AND PROTEIN IN MOUSE SKIN CARCINOGENESIS
    PATAMALAI, B
    BUROW, DL
    GIMENEZCONTI, I
    ZENKLUSEN, JC
    CONTI, CJ
    KLEINSZANTO, AJP
    FISCHER, SM
    MOLECULAR CARCINOGENESIS, 1994, 9 (04) : 220 - 229
  • [10] Cellular and extracellular biology of the latent transforming growth factor-β binding proteins
    Sinha, S
    Nevett, C
    Shuttleworth, CA
    Kielty, CM
    MATRIX BIOLOGY, 1998, 17 (8-9) : 529 - 545
12345