SOLID-PHASE SYNTHESIS AND HYBRIDIZATION PROPERTIES OF DNA CONTAINING SULFIDE-LINKED DINUCLEOSIDES

被引：33

作者：

KAWAI, SH ^{[1
]}

WANG, D ^{[1
]}

GIANNARIS, PA ^{[1
]}

DAMHA, MJ ^{[1
]}

JUST, G ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT CHEM, MONTREAL H3A 2K6, QUEBEC, CANADA

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/21.6.1473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligodeoxyribonucleotides incorporating non-hydrolyzable dialkyl sulfide linked thymidine dimers (TsT) were synthesized chemically by the solid-phase approach. The sulfide dimer TsT was stable to degradation by snake-venom phosphodiesterase, calf spleen phosphodiesterase, Nuclease P1 and Nuclease S1. Thermal denaturation analysis indicated that the incorporation of TsT dimers into DNA weakened, but did not prevent, binding to complementary DNA and RNA over a wide range of salt concentrations (10 mM to 2 M NaCl).

引用

页码：1473 / 1479

页数：7

共 41 条

[1] OLIGODEOXYNUCLEOSIDE METHYLPHOSPHONATES - SYNTHESIS AND ENZYMATIC DEGRADATION [J].

AGRAWAL, S ;

GOODCHILD, J .

TETRAHEDRON LETTERS, 1987, 28 (31) :3539-3542

[2] INFLUENCE OF THE INCORPORATION OF (S)-9-(3,4-DIHYDROXY-BUTYL)ADENINE ON THE ENZYMATIC STABILITY AND BASE-PAIRING PROPERTIES OF OLIGODEOXYNUCLEOTIDES [J].

AUGUSTYNS, K ;

VANAERSCHOT, A ;

VANSCHEPDAEL, A ;

URBANKE, C ;

HERDEWIJN, P .

NUCLEIC ACIDS RESEARCH, 1991, 19 (10) :2587-2593

[3] INHIBITION OF RABBIT GLOBIN MESSENGER-RNA TRANSLATION BY SEQUENCE-SPECIFIC OLIGODEOXYRIBONUCLEOTIDES [J].

BLAKE, KR ;

MURAKAMI, A ;

MILLER, PS .

BIOCHEMISTRY, 1985, 24 (22) :6132-6138

[4]

Cantor CR, 1980, BIOPHYSICAL CHEM

[5] CHEMICAL AND BIOCHEMICAL-STUDIES WITH DITHIOATE DNA [J].

CARUTHERS, MH ;

BEATON, G ;

CUMMINS, L ;

DELLINGER, D ;

GRAFF, D ;

MA, YX ;

MARSHALL, WS ;

SASMOR, H ;

SHANKLAND, P ;

WU, JV ;

YAU, EK .

NUCLEOSIDES & NUCLEOTIDES, 1991, 10 (1-3) :47-59

[6]

COHEN JS, 1989, TOPICS MOL STRUCTURA, V12

[7] SYNTHESIS OF HEXANUCLEOTIDE ANALOGS CONTAINING DIISOPROPYLSILYL INTERNUCLEOTIDE LINKAGES [J].

CORMIER, JF ;

OGILVIE, KK .

NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4583-4594

[8] PHYSICAL-PROPERTIES OF OLIGONUCLEOTIDES CONTAINING PHOSPHORAMIDATE-MODIFIED INTERNUCLEOSIDE LINKAGES [J].

DAGLE, JM ;

ANDRACKI, ME ;

DEVINE, RJ ;

WALDER, JA .

NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1805-1810

[9] AN IMPROVED PROCEDURE FOR DERIVATIZATION OF CONTROLLED-PORE GLASS-BEADS FOR SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS [J].

DAMHA, MJ ;

GIANNARIS, PA ;

ZABARYLO, SV .

NUCLEIC ACIDS RESEARCH, 1990, 18 (13) :3813-3821

[10] OLIGODEOXYNUCLEOTIDES CONTAINING UNNATURAL L-2'-DEOXYRIBOSE [J].

DAMHA, MJ ;

GIANNARIS, PA ;

MARFEY, P ;

REID, LS .

TETRAHEDRON LETTERS, 1991, 32 (23) :2573-2576

← 1 2 3 4 5 →