DIRECT MEASUREMENT OF THE EQUILIBRIUM BETWEEN GLUTATHIONE AND DITHIOTHREITOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：50

作者：

CHAU, MH ^{[1
]}

NELSON, JW ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV, DEPT BIOCHEM, 322 CHOPPIN HALL, BATON ROUGE, LA 70803 USA

来源：

FEBS LETTERS | 1991年 / 291卷 / 02期

关键词：

GLUTATHIONE; DITHIOTHREITOL; THIOL REAGENT; DISULFIDE EXCHANGE;

D O I：

10.1016/0014-5793(91)81305-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The equilibrium constant between reduced glutathione (GSH), oxidized glutathione (GSSG), reduced dithiothreitol (DTT(SH)SH), and oxidized dithiothreitol (DTT(S)1S) has been directly measured by high performance liquid chromatography analysis of equilibrium mixtures. The equilibrium constant at 25-degrees-C for the reaction GSSG + DTT(SH)SH half-arrow-right-over-half-arrow-left 2GSH + DTT(S)1S varies from approximately 200 M, below pH 8, to approximately 2800 M, above pH 11. The observed pH dependence is generally consistent with published values of acid dissociation constants of these thiols.

引用

页码：296 / 298

页数：3

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