STRUCTURE-FUNCTION ANALYSIS OF HUMAN INTERLEUKIN-6 - EVIDENCE FOR THE INVOLVEMENT OF THE CARBOXY-TERMINUS IN FUNCTION

被引：73

作者：

KRUTTGEN, A ^{[1
]}

ROSEJOHN, S ^{[1
]}

MOLLER, C ^{[1
]}

WROBLOWSKI, B ^{[1
]}

WOLLMER, A ^{[1
]}

MULLBERG, J ^{[1
]}

HIRANO, T ^{[1
]}

KISHIMOTO, T ^{[1
]}

HEINRICH, PC ^{[1
]}

机构：

[1] OSAKA UNIV, INST MOLEC & CELLULAR BIOL, SUITA, OSAKA 565, JAPAN

来源：

FEBS LETTERS | 1990年 / 262卷 / 02期

关键词：

Biological activity; Carboxy-terminus; Circular dichroism; Deletion mutant; Interleukin-6; Secondary structure prediction;

D O I：

10.1016/0014-5793(90)80219-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-terminally deleted analogs of human interleukin-6 (IL-6) have been constructed at the cDNA level, and after cell-free transcription and translation their biological activity was analyzed. Removal of only 4 amino acids resulted in complete loss of biological activity as determined by the B9 cell proliferation assay. Secondary structure prediction of human IL-6 resulted in 58% helix, 14% β-structure, and 28% turn and coil (average of 3 independent methods). The circular dichroism of recombinant human IL-6 was measured in the near and far UV. Evaluation of the latter in terms of secondary structures gave 67% helix, 15% β-structure, and 18% turn and coil. © 1990.

引用

页码：323 / 326

页数：4

共 23 条

[1] PRODUCTION OF HYBRIDOMA GROWTH-FACTOR BY HUMAN-MONOCYTES
AARDEN, LA
DEGROOT, ER
SCHAAP, OL
LANSDORP, PM
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1987, 17 (10) : 1411 - 1416
[2] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS
BONNER, WM
LASKEY, RA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01): : 83 - 88
[3] BRAKENHOFF JPJ, 1989, J IMMUNOL, V143, P1175
[4] Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45
[5] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS
GARNIER, J
OSGUTHORPE, DJ
ROBSON, B
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) : 97 - 120
[6] GASQUEL O, 1988, CABIOS, V4, P357
[7] CELL-FREE-SYNTHESIZED INTERLEUKIN-6 (BSF-2/IFN-BETA-2) EXHIBITS HEPATOCYTE-STIMULATING ACTIVITY
GEIGER, T
ANDUS, T
BAUER, J
NORTHOFF, H
GANTER, U
HIRANO, T
KISHIMOTO, T
HEINRICH, PC
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 175 (01): : 181 - 186
[8] O-GLYCOSYLATION AND N-GLYCOSYLATION LEAD TO DIFFERENT MOLECULAR MASS FORMS OF HUMAN MONOCYTE INTERLEUKIN-6
GROSS, V
ANDUS, T
CASTELL, J
BERG, DV
HEINRICH, PC
GEROK, W
[J]. FEBS LETTERS, 1989, 247 (02): : 323 - 326
[9] INTERLEUKIN-6 AND THE ACUTE PHASE RESPONSE
HEINRICH, PC
CASTELL, JV
ANDUS, T
[J]. BIOCHEMICAL JOURNAL, 1990, 265 (03) : 621 - 636
[10] HENIKOFF S, 1987, METHOD ENZYMOL, V155, P156

← 1 2 3 →