A COLORIMETRIC METHOD FOR ALPHA-NAPHTHOL AND ITS APPLICATION TO ASSAY OF HYDROLASES

被引：8

作者：

WEISSMANN, B

机构：

[1] Department of Biological Chemistry, University of Illinois College of Medicine, Chicago

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(69)90182-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coupling of α-naphthol with diazotized Aniline Yellow in sodium carbonate solution produces a water-soluble dye (λmax 580 mμ). The reaction serves as the basis of a sensitive estimation of α-naphthol. The general applicability of this colorimetry to assay of hydrolases, using naphthyl esters and glycosides as substrates, is illustrated by an assay of β-acetylglucosaminidase. © 1969.

引用

页码：295 / +

页数：1

共 10 条

[1] MEASUREMENT OF ACID PHOSPHATASE ACTIVITY OF URINE [J].