EFFECT OF GUANIDINIUM, CARBAMOYLGUANIDINIUM, AND GUANYLGUANIDINIUM SALTS ON SOLUBILITY OF BENZOYL-L-TYROSINE ETHYL ESTER AND ACETYLTETRAGLYCINE ETHYL ESTER IN WATER

被引：10

作者：

CASTELLI.FJ

BARKER, R

机构：

[1] Department of Biochemistry, The University of Iowa, Iowa City

[2] Department of Biochemistry, Duke University Medical Center, Durham, N. C.

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 08期

关键词：

D O I：

10.1021/bi00836a046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the chloride, bromide, iodide, and thiocyanate salts of guanidinium, carbamoylguanidinium, and guanylguanidinium cations on the water solubility of benzoyl-L-tyrosine ethyl ester, a model hydrophobic compound, and acetyltetraglycine ethyl ester, a model peptide and amide compound, have been examined. Regardless of the cation used, the solubility of both model compounds increases progressively through the series chloride < bromide < iodide < thiocyanate. This anion series parallels the effectiveness of these anions as denaturants of several proteins. When the anion is held conslant, the cation effect on the solubility of benzoyl-L-tyrosine ethyl ester is guanidinium < guanylguanidinium < carbamoylguanidinium and the cation effect on the solubility of acetyltetraglycine ethyl ester is guanidinium < carbamoylguanidinium < guanylguanidinium. It is concluded that the protein denaturing effectiveness of these guanidinium, carbamoylguanidinium, and guanylguanidinium salts is due to their ability to increase the solubility of protein hydrophobic and peptide amide groups. © 1969, American Chemical Society. All rights reserved.

引用

页码：3439 / &

共 16 条

[1] FUNCTIONAL GROUPS IN GELATION OF GELATIN [J].