ISOLATION OF AUTOPHAGOCYTOSIS MUTANTS OF SACCHAROMYCES-CEREVISIAE

被引：476

作者：

THUMM, M ^{[1
]}

EGNER, R ^{[1
]}

KOCH, B ^{[1
]}

SCHLUMPBERGER, M ^{[1
]}

STRAUB, M ^{[1
]}

VEENHUIS, M ^{[1
]}

WOLF, DH ^{[1
]}

机构：

[1] UNIV GRONINGEN, CTR BIOL, ELECTRON MICROSCOPY LAB, HAREN, NETHERLANDS

来源：

FEBS LETTERS | 1994年 / 349卷 / 02期

关键词：

YEAST; VACUOLE; AUTOPHAGOCYTOSIS; SCREENING PROCEDURE;

D O I：

10.1016/0014-5793(94)00672-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein degradation in the vacuole (lysosome) is an important event in cellular regulation. In yeast, as in mammalian cells, a major route of protein uptake for degradation into the vacuole (lysosome) has been found to be autophagocytosis. The discovery of this process in yeast enables the elucidation of its mechanisms via genetic and molecular biological investigations. Here we report the isolation of yeast mutants defective in autophagocytosis (aut mutants), using a rapid colony screening procedure.

引用

页码：275 / 280

页数：6

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