INCREASED RESISTANCE TO POTATO VIRUS-X AND PRESERVATION OF CULTIVAR PROPERTIES IN TRANSGENIC POTATO UNDER FIELD CONDITIONS

被引:54
作者
JONGEDIJK, E
DESCHUTTER, AAJM
STOLTE, T
VANDENELZEN, PJM
CORNELISSEN, BJC
机构
[1] DESCHUTIER FARMING PARTNERSHIP,8256 RV BIDDINGHUIZEN,NETHERLANDS
[2] DUTCH INSPECT SERV,FDN INSPECT SERV NOORDZEEPOLDERS,7801 BC EMMELOORD,NETHERLANDS
来源
BIO-TECHNOLOGY | 1992年 / 10卷 / 04期
关键词
D O I
10.1038/nbt0492-422
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
During the last three years we performed field trials to assess levels of resistance against potato virus X (PVX) and changes in intrinsic properties of the potato cultivars Bintje and Escort upon the introduction of the PVX coat protein (CP) gene. Analysis of leaf and tuber samples collected in the field at two week intervals revealed a stable expression of the PVX CP gene throughout the growing season. This resulted in a large decrease in PVX incidence among clonal progeny obtained from previously infected Bintje and Escort clones. Based on evaluation of 50 defined morphological characteristics, tuber yield and grading, 81.8% of the Escort and 17.9% of the Bintje derived transgenic clones proved to be true to type. Overall lightsprout morphology was a useful criterion for the early detection of deviant transgenic clones. Using the polymerase chain reaction (PCR) with convergent primers spanning transgenic sequences, true to type clones could be distinguished unambiguously from the corresponding untransformed cultivars. Clear distinctions between independent transgenic clones could be made by inverted PCR (IPCR) diagnosis revealing integration-specific border fragments. These results demonstrate the commercial feasibility of improving potato cultivars by selectively adding new traits while preserving intrinsic properties, and the possibility of unambiguously identifying independent transgenic cultivars.
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页码:422 / 429
页数:8
相关论文
共 28 条
[1]  
[Anonymous], 1971, STAT PRINCIPLES EXPT
[2]   COAT PROTEIN-MEDIATED RESISTANCE AGAINST VIRUS-INFECTION [J].
BEACHY, RN ;
LOESCHFRIES, S ;
TUMER, NE .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1990, 28 :451-474
[3]  
Beemster A, 1987, VIRUSES POTATOES SEE, P84
[4]   CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES [J].
CLARK, MF ;
ADAMS, AN .
JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) :475-483
[5]   VIRAL PROTECTION IN TRANSGENIC TOBACCO PLANTS EXPRESSING THE CUCUMBER MOSAIC-VIRUS COAT PROTEIN OR ITS ANTISENSE RNA [J].
CUOZZO, M ;
OCONNELL, KM ;
KANIEWSKI, W ;
FANG, RX ;
CHUA, NH ;
TUMER, NE .
BIO-TECHNOLOGY, 1988, 6 (05) :549-&
[6]   A QUICK METHOD TO ESTIMATE THE T-DNA COPY NUMBER IN TRANSGENIC PLANTS AT AN EARLY STAGE AFTER TRANSFORMATION, USING INVERSE PCR [J].
DOES, MP ;
DEKKER, BMM ;
DEGROOT, MJA ;
OFFRINGA, R .
PLANT MOLECULAR BIOLOGY, 1991, 17 (01) :151-153
[7]  
Harris P. M., 1978, POTATO CROP SCI BASI
[8]   ANALYSIS OF THE MECHANISM OF PROTECTION IN TRANSGENIC PLANTS EXPRESSING THE POTATO VIRUS-X COAT PROTEIN OR ITS ANTISENSE RNA [J].
HEMENWAY, C ;
FANG, RX ;
KANIEWSKI, WK ;
CHUA, NH ;
TUMER, NE .
EMBO JOURNAL, 1988, 7 (05) :1273-1280
[9]   THE DEVELOPMENT OF VIRUS-RESISTANT ALFALFA, MEDICAGO-SATIVA L [J].
HILL, KK ;
JARVISEAGAN, N ;
HALK, EL ;
KRAHN, KJ ;
LIAO, LW ;
MATHEWSON, RS ;
MERLO, DJ ;
NELSON, SE ;
RASHKA, KE ;
LOESCHFRIES, LS .
BIO-TECHNOLOGY, 1991, 9 (04) :373-377
[10]   THE GENETIC-ENGINEERING OF 2 COMMERCIAL POTATO CULTIVARS FOR RESISTANCE TO POTATO VIRUS-X [J].
HOEKEMA, A ;
HUISMAN, MJ ;
MOLENDIJK, L ;
VANDENELZEN, PJM ;
CORNELISSEN, BJC .
BIO-TECHNOLOGY, 1989, 7 (03) :273-278