BIOLUMINESCENT LISTERIA-MONOCYTOGENES PROVIDE A RAPID ASSAY FOR MEASURING BIOCIDE EFFICACY

被引：0
|
作者：
WALKER, AJ
JASSIM, SAA
HOLAH, JT
DENYER, SP
STEWART, GSAB
机构：
[1] CAMPDEN FOOD & DRINK RES ASSOC, DIV MICROBIOL & FOOD HYG, CHIPPING CAMPDEN, GLOS, ENGLAND
[2] BRIGHTON POLYTECH, DEPT PHARM, BRIGHTON BN2 4AT, E SUSSEX, ENGLAND
来源：
FEMS MICROBIOLOGY LETTERS | 1992年 / 91卷 / 03期
关键词：
BIOLUMINESCENCE; LISTERIA-MONOCYTOGENES; PHENOL; CHLORHEXIDINE DIACETATE;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A recombinant derivative of Listeria monocytogenes 23074, engineered to express the luxAB genes of Vibrio fischeri MJ1, has a bioluminescent phenotype that provides a rapid monitor of microbial viability. The antibacterial activity of phenol and chlorhexidine diacetate (Hibitane) was measured using both bioluminescence and viable counts. Concentration exponents were assessed as 7.3 for phenol and 2.63 for chlorhexidine diacetate using bioluminescence, and 8.3 and 2.13 respectively using plate counts. The rapidity of bioluminescence measurement constitutes a major advantage in biocide assessment.
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页码：251 / 256
页数：6
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